Colleagues

Vilmos Tubak M.D., Ph. D. – Chief Executive Officer
Mária A. Deli M.D.,Ph.D. – Scientific Advisor
Szilvia Veszelka Ph.D.-Chief Scientific Officer

 

 

Consultancy and Services

We offer consultancy and services as follows:

Molecular biology: standard molecular cloning; PCR, RT-PCR, Quantitative real-time PCR diagnostics; RNAi technology, production of knock-down mammalian cell lines

 

Biochemistry: production of recombinant proteins; protein and protein complex purification (immunoaffinity, FPLC (Fast Pressure Liquid Chromatography), in vivo cross-linking); Western blotting; immunohistochemistry; immunofluorescence; immunoprecipitation

 

Cell biology: production of transgenic mice by the pronuclear injection of cloned DNA sequences; mouse embryonic stem cell culture; gene-targeting in mouse embryonic stem cells; production of knock-out mice; standard mammalian cell culture, transfection and manipulation; production of transgenic mammalian cell lines; isolation and culture of adult mesenchymal stem cells and embryonic stem cells; molecular characterization of stem cell lines by FACS (Fluorescence Activated Cell Sorting)

 

Cytology: FISH (Fluorescent In Situ Hybridisation) analysis; immunofluorescence; indirect immunostaining

 

Cell toxicity tests: colorimetric endpoint assays, cell cycle and apoptosis analysis by FACS, double staining of cell nucleus for detection of live/dead cells by confocal microscopy, kinetic analysis of cell toxicity by real-time cell electronic sensing (RT-CES) based on impedance

 

Measurement of compound permeability across biological barriers: testing the bidirectional permeability of compounds and drug candidates across culture models of different endothelial and epithelial barriers

 

Cell culture models of biological barriers: blood-brain, intestinal, nasal and lung barrier co-culture models

 

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Products

KeraE6 immortalized human keratinocyte cell line with normal chromosome number.

Therbiogen distributes the products of PharmaCo-Cell Ltd.  in Europe:
For a full list of products see: http://www.pharmacocell.co.jp

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